Beta-arrestin2 K11,12R GFP
(Plasmid
#35412)
-
Depositing Lab
-
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 35412 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepEGFP-N1
-
Backbone manufacturerClontech
- Backbone size w/o insert (bp) 4700
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameβarrestin2
-
SpeciesR. norvegicus (rat)
-
Insert Size (bp)1500
-
MutationK11,12R
-
Entrez GeneArrb2 (a.k.a. BARRES)
- Promoter CMV
-
Tag
/ Fusion Protein
- EGFP (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site ApaI (unknown if destroyed)
- 5′ sequencing primer n/a
- 3′ sequencing primer EGFP-N (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
Beta-arrestin2 K11,12R GFP was a gift from Robert Lefkowitz (Addgene plasmid # 35412 ; http://n2t.net/addgene:35412 ; RRID:Addgene_35412) -
For your References section:
Receptor-specific ubiquitination of beta-arrestin directs assembly and targeting of seven-transmembrane receptor signalosomes. Shenoy SK, Lefkowitz RJ. J Biol Chem. 2005 Apr 15;280(15):15315-24. Epub 2005 Feb 7. 10.1074/jbc.M412418200 PubMed 15699045