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Addgene

Beta-arrestin2 GFP WT
(Plasmid #35411)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 35411 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-N1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    βarrestin2
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    1500
  • Entrez Gene
    Arrb2 (a.k.a. BARRES)
  • Promoter CMV
  • Tag / Fusion Protein
    • EGFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site ApaI (not destroyed)
  • 5′ sequencing primer n/a
  • 3′ sequencing primer EGFP-N
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Beta-arrestin2 GFP WT was a gift from Robert Lefkowitz (Addgene plasmid # 35411 ; http://n2t.net/addgene:35411 ; RRID:Addgene_35411)

  • For your References section:

    Receptor-specific ubiquitination of beta-arrestin directs assembly and targeting of seven-transmembrane receptor signalosomes. Shenoy SK, Lefkowitz RJ. J Biol Chem. 2005 Apr 15;280(15):15315-24. Epub 2005 Feb 7. 10.1074/jbc.M412418200 PubMed 15699045
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