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Addgene

Arrb1-Q394L
(Plasmid #35402)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 35402 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5428
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    beta-arrestin-1
  • Alt name
    Arrb1
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    1357
  • Mutation
    Q394L
  • Entrez Gene
    Arrb1 (a.k.a. BARRES)
  • Promoter CMV
  • Tag / Fusion Protein
    • Flag (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site None (not destroyed)
  • 3′ cloning site None (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer BGH-Rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Arrb1-Q394L was a gift from Robert Lefkowitz (Addgene plasmid # 35402 ; http://n2t.net/addgene:35402 ; RRID:Addgene_35402)

  • For your References section:

    A stress response pathway regulates DNA damage through beta2-adrenoreceptors and beta-arrestin-1. Hara MR, Kovacs JJ, Whalen EJ, Rajagopal S, Strachan RT, Grant W, Towers AJ, Williams B, Lam CM, Xiao K, Shenoy SK, Gregory SG, Ahn S, Duckett DR, Lefkowitz RJ. Nature. 2011 Aug 21;477(7364):349-53. doi: 10.1038/nature10368. 10.1038/nature10368 PubMed 21857681
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