MSCV-Myc-IRES-GFP
(Plasmid #35396)

• Depositing Lab: Martine Roussel
• Publication: Kawauchi et al Cancer Cell. 2012 Feb 14;21(2):168-80.

Backbone

• Vector backbone: MSCV
• Vector type: Mammalian Expression, Retroviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL1 Blue
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: Myc
• Alt name: C-Myc
• Species: M. musculus (mouse)
• Insert Size (bp): 1346
• GenBank ID: NM_010849
• Entrez Gene: Myc (a.k.a. Myc2, Niard, Nird, bHLHe39)

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (unknown if destroyed)
• 3′ cloning site: XhoI (unknown if destroyed)
• 5′ sequencing primer: 5’ GGATCCACGACGATGCCCCTCAACGTGAAC

Gene/Insert 2

• Gene/Insert name: GFP
• Alt name: green fluorescent protein
• Species: Aequorea Victoria

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry