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Addgene

MSCV-Myc-IRES-RFP
(Plasmid #35395)

Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 35395 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    MSCV
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    RFP

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    Myc
  • Alt name
    C-Myc
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1346
  • GenBank ID
    NM_010849
  • Entrez Gene
    Myc (a.k.a. Myc2, Niard, Nird, bHLHe39)

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)
  • 5′ sequencing primer 5' GGATCCACGACGATGCCCCTCAACGTGAAC
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    RFP
  • Alt name
    red fluorescent protein
  • Species
    Discosoma

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MSCV-Myc-IRES-RFP was a gift from Martine Roussel (Addgene plasmid # 35395 ; http://n2t.net/addgene:35395 ; RRID:Addgene_35395)
  • For your References section:

    A mouse model of the most aggressive subgroup of human medulloblastoma. Kawauchi D, Robinson G, Uziel T, Gibson P, Rehg J, Gao C, Finkelstein D, Qu C, Pounds S, Ellison DW, Gilbertson RJ, Roussel MF. Cancer Cell. 2012 Feb 14;21(2):168-80. 10.1016/j.ccr.2011.12.023 PubMed 22340591