FLAG-p54
(Plasmid #35379)

• Depositing Lab: Benjamin Blencowe
• Publication: Rosonina et al Mol Cell Biol. 2005 Aug;25(15):6734-46.

Backbone

• Vector backbone: pCMVmyc
• Backbone size w/o insert (bp): 3800
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: p54
• Alt name: p54(nrb)/NonO
• Species: H. sapiens (human)
• Mutation: A464V
• Entrez Gene: NONO (a.k.a. MRXS34, NMT55, NRB54, P54, P54NRB, PPP1R114)
• Promoter: CMV
• Tag / Fusion Protein:
  • FLAG (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (unknown if destroyed)
• 3′ cloning site: XhoI (unknown if destroyed)
• 5′ sequencing primer: CMV-Fwd

Resource Information

• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry