Myc-PSFΔRRM2
(Plasmid #35377)

• Depositing Lab: Benjamin Blencowe
• Publication: Rosonina et al Mol Cell Biol. 2005 Aug;25(15):6734-46.

Backbone

• Vector backbone: pCMVmyc
• Backbone size w/o insert (bp): 3800
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PSF
• Alt name: SFPQ
• Species: H. sapiens (human)
• Mutation: Deletion of residues 375-429
• Entrez Gene: SFPQ (a.k.a. POMP100, PPP1R140, PSF)
• Promoter: CMV
• Tag / Fusion Protein:
  • Myc (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NsiI (unknown if destroyed)
• 3′ cloning site: SpeI (unknown if destroyed)
• 5′ sequencing primer: CMV-Fwd

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry