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pBbE1k-RFP
(Plasmid #35333)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 35333 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBb
  • Backbone size w/o insert (bp) 3528
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RFP
  • Alt name
    JBEI Part ID: JPUB_000099
  • Species
    Synthetic
  • Insert Size (bp)
    678
  • Promoter Trc

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ sequencing primer jkRFP-R (gctttggaaccgtactggaa)
  • 3′ sequencing primer jkRFP-F (tggtcactacgacgctgaag)
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

JBEI Part ID: JPUB_000099; Origin: colE1

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBbE1k-RFP was a gift from Jay Keasling (Addgene plasmid # 35333 ; http://n2t.net/addgene:35333 ; RRID:Addgene_35333)

  • For your References section:

    BglBrick vectors and datasheets: A synthetic biology platform for gene expression. Lee TS, Krupa RA, Zhang F, Hajimorad M, Holtz WJ, Prasad N, Lee SK, Keasling JD. J Biol Eng. 2011 Sep 20;5:12. 10.1186/1754-1611-5-12 PubMed 21933410
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