Myc-PSFΔRRM1+2
(Plasmid
#35267)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 35267 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCMVmyc
- Backbone size w/o insert (bp) 3800
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePSF
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Alt nameSFPQ
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SpeciesH. sapiens (human)
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MutationDeletion of residues 298-429
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Entrez GeneSFPQ (a.k.a. POMP100, PPP1R140, PSF)
- Promoter CMV
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Tag
/ Fusion Protein
- Myc (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NsiI (unknown if destroyed)
- 3′ cloning site SpeI (unknown if destroyed)
- 5′ sequencing primer CMV-Fwd (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Myc-PSFΔRRM1+2 was a gift from Benjamin Blencowe (Addgene plasmid # 35267 ; http://n2t.net/addgene:35267 ; RRID:Addgene_35267) -
For your References section:
Role for PSF in mediating transcriptional activator-dependent stimulation of pre-mRNA processing in vivo. Rosonina E, Ip JY, Calarco JA, Bakowski MA, Emili A, McCracken S, Tucker P, Ingles CJ, Blencowe BJ. Mol Cell Biol. 2005 Aug;25(15):6734-46. 10.1128/MCB.25.15.6734-6746.2005 PubMed 16024807