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Addgene

humanMER20MER39
(Plasmid #35256)

Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 35256 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    PGL4.70
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 4000
  • Vector type
    Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10/P3
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    MER20/MER39
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    700
  • Promoter dPRL promoter

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NHEI (unknown if destroyed)
  • 3′ cloning site BGLII (unknown if destroyed)
  • 5′ sequencing primer GAGGAGGCTAGCGGTTTTTCAAAAGCAGCACTAC
  • 3′ sequencing primer CTCCTCAGATCTTGTAGGGGAGAAGAGATTCC
  • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    humanMER20MER39 was a gift from Gunter Wagner (Addgene plasmid # 35256 ; http://n2t.net/addgene:35256 ; RRID:Addgene_35256)
  • For your References section:

    Transformation of a transposon into a derived prolactin promoter with function during human pregnancy. Emera D, Wagner GP. Proc Natl Acad Sci U S A. 2012 Jul 10;109(28):11246-51. doi: 10.1073/pnas.1118566109. Epub 2012 Jun 25. 10.1073/pnas.1118566109 PubMed 22733751