pBID-UASC-MRG
(Plasmid #35205)

• Purpose: (Empty Backbone)
• Depositing Lab: Brian McCabe
• Publication: Wang et al PLoS One. 2012;7(7):e42102. Epub 2012 Jul 25.

Backbone

• Vector backbone: pBID-UASC-MRG
• Backbone manufacturer: Ji-Wu Wang and Brian McCabe
• Backbone size (bp): 12020
• Vector type: Drosophila Transgene Expression
• Promoter: Drosophila Synthetic Core Promoter (DSCP)
• Tags / Fusion Proteins:
  • 3XMyc (N terminal on backbone)
  • tRFP (N terminal on backbone)

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol and Ampicillin, 25 & 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): ccdB Survival
• Copy number: Unknown

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Generation of ϕC31 transgenic Drosophila for GAL4 driven expression of myc tagRFP (tRFP) fusion transgenes. Gateway cloning cassette is flanked by gypsy insulator sequences to allow uniform transgene expression between insertion sites. 10 UAS binding sites, Drosophila Synthetic Core promoter (DSCP). myc-tRFP is fused to the Nterminus of genes introduced by gateway. Transgene selection using white gene. loxP site facilitates elimination of transgene markers via Cre recombinasemediated excision with ZH-attP landing sites. Ampicillin resistant. Chloramphenicol resistant for amplification.