Skip to main content
Addgene

pBID-UAS-GGi
(Plasmid #35199)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 35199 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pBID-UAS-GGi
  • Backbone manufacturer
    Ji-Wu Wang and Brian McCabe
  • Backbone size (bp) 13081
  • Vector type
    Drosophila Transgene Expression
  • Promoter UAS

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin, 25 & 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    Unknown

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Generation of ϕC31 transgenic Drosophila for GAL4 driven RNA inhibition (RNAi). 2 inverted gateway cloning cassettes are flanked by gypsy insulator sequences to allow uniform transgene expression between insertion sites. 10 UAS binding sites, hsp70 basal promoter. ftz intron facilitates RNA hairpin formation and expression. Transgene selection using white gene. loxP site facilitates elimination of
transgene markers via Cre recombinasemediated
excision with ZH-attP landing sites. Ampicillin resistant. Chloramphenicol resistant for amplification.

Please note that the inverted sequences present can cause rearrangements of this vector. Recommend miniprep only and confirm plasmid map subsequent to amplification or cloning. After gateway recombination, recommend amplification in Stbl2 cells [Invitrogen Cat. 10268-019] and combining minipreps for Drosophila injection.

Addgene's sequencing results indicated a single nucleotide mismatch at bp# 5286 when compared to the full plasmid sequence provided by the depositing laboratory. This difference does not affect plasmid function.

Please see the associated publication for more information: Wang J-W, Beck ES, McCabe BD (2012) A Modular Toolset for Recombination Transgenesis and Neurogenetic Analysis of Drosophila. PLoS ONE 7(7): e42102 http://www.plosone.org/article/info:doi%2F10.1371%2Fjournal.pone.0042102

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBID-UAS-GGi was a gift from Brian McCabe (Addgene plasmid # 35199 ; http://n2t.net/addgene:35199 ; RRID:Addgene_35199)

  • For your References section:

    A modular toolset for recombination transgenesis and neurogenetic analysis of Drosophila. Wang JW, Beck ES, McCabe BD. PLoS One. 2012;7(7):e42102. Epub 2012 Jul 25. 10.1371/journal.pone.0042102 PubMed 22848718
Related items:
Commonly requested with: