pBID-G
(Plasmid
#35195)
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Purpose(Empty Backbone)
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 35195 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBID-G
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Backbone manufacturerJi-Wu Wang and Brian McCabe
- Backbone size (bp) 10033
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Vector typeDrosophila Transgene Expression
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberUnknown
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Generation of ϕC31 transgenic Drosophila. Gateway cloning cassette is flanked by gypsy insulator sequences to allow uniform transgene expression between insertion sites. Transgene selection using white gene. loxP site facilitates elimination of transgene markers via Cre recombinase-mediated excision with ZH-attP landing sites. Ampicillin resistant. Chloramphenicol resistant for amplification.
Please see the associated publication for more information: Wang J-W, Beck ES, McCabe BD (2012) A Modular Toolset for Recombination Transgenesis and Neurogenetic Analysis of Drosophila. PLoS ONE 7(7): e42102 http://www.plosone.org/article/info:doi%2F10.1371%2Fjournal.pone.0042102
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBID-G was a gift from Brian McCabe (Addgene plasmid # 35195 ; http://n2t.net/addgene:35195 ; RRID:Addgene_35195) -
For your References section:
A modular toolset for recombination transgenesis and neurogenetic analysis of Drosophila. Wang JW, Beck ES, McCabe BD. PLoS One. 2012;7(7):e42102. Epub 2012 Jul 25. 10.1371/journal.pone.0042102 PubMed 22848718
Map uploaded by the depositor.
