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Addgene

pLDPuro-MmnSR100N
(Plasmid #35171)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 35171 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pLDPuro-TcVAPrtTA
  • Backbone size w/o insert (bp) 10460
  • Modifications to backbone
    Tet components not present
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Srrm4
  • Alt name
    nSR100
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1800
  • Entrez Gene
    Srrm4 (a.k.a. 1500001A10Rik, B230202K19Rik, bv, mKIAA1853, nSR100)
  • Tag / Fusion Protein
    • Flag (N terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Addgene sequencing found an additional N-term FLAG tag not present in the author's sequence--this does not affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLDPuro-MmnSR100N was a gift from Benjamin Blencowe (Addgene plasmid # 35171 ; http://n2t.net/addgene:35171 ; RRID:Addgene_35171)
  • For your References section:

    Cross-regulation between an alternative splicing activator and a transcription repressor controls neurogenesis. Raj B, O'Hanlon D, Vessey JP, Pan Q, Ray D, Buckley NJ, Miller FD, Blencowe BJ. Mol Cell. 2011 Sep 2;43(5):843-50. 10.1016/j.molcel.2011.08.014 PubMed 21884984