pLDPuro-MmnSR100N
(Plasmid
#35171)
-
Depositing Lab
-
Publication
-
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 35171 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepLDPuro-TcVAPrtTA
- Backbone size w/o insert (bp) 10460
-
Modifications to backboneTet components not present
-
Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature30°C
-
Growth Strain(s)Stbl3
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameSrrm4
-
Alt namenSR100
-
SpeciesM. musculus (mouse)
-
Insert Size (bp)1800
-
Entrez GeneSrrm4 (a.k.a. 1500001A10Rik, B230202K19Rik, bv, mKIAA1853, nSR100)
-
Tag
/ Fusion Protein
- Flag (N terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer EF1a (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Addgene sequencing found an additional N-term FLAG tag not present in the author's sequence--this does not affect plasmid function.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pLDPuro-MmnSR100N was a gift from Benjamin Blencowe (Addgene plasmid # 35171 ; http://n2t.net/addgene:35171 ; RRID:Addgene_35171) -
For your References section:
Cross-regulation between an alternative splicing activator and a transcription repressor controls neurogenesis. Raj B, O'Hanlon D, Vessey JP, Pan Q, Ray D, Buckley NJ, Miller FD, Blencowe BJ. Mol Cell. 2011 Sep 2;43(5):843-50. 10.1016/j.molcel.2011.08.014 PubMed 21884984
