HBM-Luc
(Plasmid #35155)

• Depositing Lab: Linda Penn
• Publication: Facchini et al Mol Cell Biol. 1997 Jan;17(1):100-14.

Backbone

• Vector backbone: pGL2-basic
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 5597
• Vector type: Mammalian Expression, Luciferase

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Myc promoter
• Species: H. sapiens (human)
• Insert Size (bp): 2500
• Mutation: Contains 2.5 kb of the c-myc minimal promoter
• Entrez Gene: MYC (a.k.a. MRTL, MYCC, bHLHe39, c-Myc)
• Promoter: c-myc
• Tag / Fusion Protein:
  • Luciferase (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (not destroyed)
• 3′ cloning site: SacI (not destroyed)
• 5′ sequencing primer: EBV-rev
• 3′ sequencing primer: Luc_N_Rev

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Original construct in pBluescript was from E. Laufer
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid is a c-myc promoter-luciferase reporter construct designed to examine the regulation of transcription in response to conditional and constitutive Myc activation and functions as described in the associated publication.

Addgene's sequencing results identified a V13C mutation in exon 1 of c-myc when compared to GenBank reference sequence AAA59886.1