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pBbA5c-MevT-MBIS
(Plasmid #35150)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 35150 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBbA5c
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH10B
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    AtoB(o)-HMGS(o)-HMGR(o)-MK(o)-PMK(o)-PMD-Idi-IspA
  • Species
    S. cerevisiae (budding yeast); E. Coli
  • Promoter LacUV5,

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR1, BglII (not destroyed)
  • 3′ cloning site BamH1, Xho1 (unknown if destroyed)
  • 5′ sequencing primer gatcgtttaggcacccca
  • 3′ sequencing primer CGTTTTATTTGATGCCTGG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBbA5c-MevT-MBIS was a gift from Jay Keasling & Taek Soon Lee (Addgene plasmid # 35150 ; http://n2t.net/addgene:35150 ; RRID:Addgene_35150)

  • For your References section:

    Identification and microbial production of a terpene-based advanced biofuel. Peralta-Yahya PP, Ouellet M, Chan R, Mukhopadhyay A, Keasling JD, Lee TS. Nat Commun. 2011 Sep 27;2:483. doi: 10.1038/ncomms1494. 10.1038/ncomms1494 PubMed 21952217
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