pAG61
(Plasmid #35129)

• Depositing Lab: John McCusker
• Publication: Goldstein et al Yeast. 1999 Oct;15(14):1541-53.

Backbone

• Vector backbone: pFA6
• Modifications to backbone: Transformed E. coli cells are resistant to ampicillin. The plasmid contains the URA3 gene from Candida albicans and confers uracil prototrophy of transformed auxotrophic yeasts. In contrast to pAG60 this plasmid contains direct repeats of 500 bp from Aschbya gossypii flanking the insert. Plasmid usefull as template for PCR to create gene deletions. Integrative transformation of the respective PCR fragment and selection of the mutants. Provides the possiblity for a marker rescue by homologous recombination at the direct repeats. Counterselection with floro orotic acid.
• Vector type: Yeast replicating
• Selectable markers: C.albicans URA3

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: URA3
• Species: Candida albicans

Resource Information

• Supplemental Documents:
  • Sequence file in plain text format
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry