pAG26
(Plasmid #35127)

• Depositing Lab: John McCusker
• Publication: Goldstein et al Yeast. 1999 Oct;15(14):1541-53.

Backbone

• Vector backbone: pRS 316
• Modifications to backbone: Transformed E.coli cells are resistant to ampicillin. The plasmid contains the hph gene from Klebsiella pneumoniae encoding hygromycin B phosphotransferase and confers resistance to the antibiotic hygromycin B of transformed yeasts. In contrast to pAG32 and pAG34 this plasmid contains the elements to maintain the centromeric plasmid. Transformation marker is the URA3 gene.
• Vector type: Yeast replicating
• Selectable markers: Hygromycin B

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: hph
• Species: Klebsiella pneumoniae

Resource Information

• Supplemental Documents:
  • sequence file in plain text format
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry