pAG36
(Plasmid #35126)

• Depositing Lab: John McCusker
• Publication: Goldstein et al Yeast. 1999 Oct;15(14):1541-53.

Backbone

• Vector backbone: pRS316
• Modifications to backbone: Transformed E.coli cells are resistant to ampicillin. The plasmid contains the nat1 gene from Streptomyces noursei encoding nourseothricin N-acetyl-transferase and confers resistance to the antibiotic nourseothricin of transformed yeasts. In contrast to pAG25 and pAG35 this plasmid contains the elements to maintain the centromeric plasmid. Transformation marker is the URA3 gene.
• Vector type: Yeast replicating
• Selectable markers: Nourseothricin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: nat1
• Species: Streptomyces noursei

Resource Information

• Supplemental Documents:
  • Sequence file in plain text format
• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry