-
Depositing Lab
-
Publication
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 35124 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepFA6
-
Modifications to backboneTransformed E.coli cells are resistant to ampicillin. The plasmid contains the pat1 gene from Streptomyces viridochromogenes encoding phosphinothricin N-acetyl-transferase and confers resistance to the antibiotic bialaphos of transformed yeasts. Other plasmids conferring bialaphos resistance: pAG29. Plasmid usefull as template for PCR to create gene deletions. Integrative transformation of the respective PCR fragment and selection of the mutants. The plasmid provides the possibility for a marker rescue by homologous recombination at the direct repeats.
-
Vector typeYeast replicating
-
Selectable markersBialaphos
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert namepatMX3
-
SpeciesStreptomyces viridochromogenes
Resource Information
-
Supplemental Documents
-
Articles Citing this Plasmid
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pAG31 was a gift from John McCusker (Addgene plasmid # 35124 ; http://n2t.net/addgene:35124 ; RRID:Addgene_35124) -
For your References section:
Three new dominant drug resistance cassettes for gene disruption in Saccharomyces cerevisiae. Goldstein AL, McCusker JH. Yeast. 1999 Oct;15(14):1541-53. 10.1002/(SICI)1097-0061(199910)15:14<1541::AID-YEA476>3.0.CO;2-K PubMed 10514571