pAG35
(Plasmid #35123)

• Depositing Lab: John McCusker
• Publication: Goldstein et al Yeast. 1999 Oct;15(14):1541-53.

Backbone

• Vector backbone: pFA6
• Modifications to backbone: Transformed E.coli cells are resistant to ampicillin. The plasmids contains the nat1 gene from Streptomyces noursei encoding nourseothricin N-acetyl-transferase and confers resistance to the antibiotic nourseothricin of transformed yeasts. In contrast to pAG25 this plasmid contains direct repeats of 500 bp from Aschbya gossypii flanking the insert. Plasmid usefull as template for PCR to create gene deletions. Integrative transformation of the respective PCR fragment and selection of the mutants. Provides the possiblity for a marker rescue by homologous recombination at the direct repeats
• Vector type: Yeast replicating
• Selectable markers: Nourseothricin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: natMX3
• Species: Streptomyces noursei

Resource Information

• Supplemental Documents:
  • Sequence file in plain text format

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry