pAG25
(Plasmid #35121)

• Depositing Lab: John McCusker
• Publication: Goldstein et al Yeast. 1999 Oct;15(14):1541-53.

Backbone

• Vector backbone: pFA6
• Modifications to backbone: Transformed E.coli cells are resistant to ampicillin. The plasmid contains the nat1 gene from Streptomyces noursei encoding nourseothricin N-acetyl-transferase and confers resistance to the antibiotic nourseothricin of transformed yeasts. Other plasmids conferring nourseothricin resistance: pAG35, pAG36. Plasmid usefull as template for PCR to create gene deletions. Integrative transformation of the respective PCR fragment and selection of the mutants.
• Vector type: Yeast replicating
• Selectable markers: Nourseothricin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: natMX4
• Species: Streptomyces noursei

Resource Information

• Supplemental Documents:
  • sequence file in plain text format
• Articles Citing this Plasmid:
  • 10 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry