pBN27
(Plasmid
#35120)
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 35120 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCR2.1-TOPO
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Vector typeYeast replicating
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namefcy1delta1
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SpeciesS. cerevisiae (budding yeast)
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Mutationamplicon has a complete deletion of FCY1 ORF, flanked by ~500bp of sequence upstream and downstream of FCY1 ORF
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBN27 was a gift from John McCusker (Addgene plasmid # 35120 ; http://n2t.net/addgene:35120 ; RRID:Addgene_35120) -
For your References section:
Cytosine deaminase MX cassettes as positive/negative selectable markers in Saccharomyces cerevisiae. Hartzog PE, Nicholson BP, McCusker JH. Yeast. 2005 Jul 30;22(10):789-98. 10.1002/yea.1245 PubMed 16088873