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Addgene

pBN27
(Plasmid #35120)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 35120 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCR2.1-TOPO
  • Vector type
    Yeast replicating

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    fcy1delta1
  • Species
    S. cerevisiae (budding yeast)
  • Mutation
    amplicon has a complete deletion of FCY1 ORF, flanked by ~500bp of sequence upstream and downstream of FCY1 ORF

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBN27 was a gift from John McCusker (Addgene plasmid # 35120 ; http://n2t.net/addgene:35120 ; RRID:Addgene_35120)

  • For your References section:

    Cytosine deaminase MX cassettes as positive/negative selectable markers in Saccharomyces cerevisiae. Hartzog PE, Nicholson BP, McCusker JH. Yeast. 2005 Jul 30;22(10):789-98. 10.1002/yea.1245 PubMed 16088873
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