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Addgene

pSA25
(Plasmid #35116)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 35116 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRS316
  • Modifications to backbone
    Transformed E. coli cells are resistant to ampicillin. The plasmid has been used to construct the plasmids pSA39, pSA40, pSA47, and pSA48. It contains the LYS5MX4 gene as marker for deletion of yeast genes and the URA3 gene as yeast transformation marker. Yeast shuttle plasmid with CEN6/ARSH4 region.
  • Vector type
    Yeast replicating
  • Selectable markers
    Lysine,URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    lys5MX4
  • Species
    S. cerevisiae (budding yeast)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSA25 was a gift from John McCusker (Addgene plasmid # 35116 ; http://n2t.net/addgene:35116 ; RRID:Addgene_35116)

  • For your References section:

    Positive and negative selection LYS5MX gene replacement cassettes for use in Saccharomyces cerevisiae. Ito-Harashima S, McCusker JH. Yeast. 2004 Jan 15;21(1):53-61. 10.1002/yea.1057 PubMed 14745782
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