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Addgene

pSA40
(Plasmid #35114)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 35114 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFA6-kanMX4
  • Vector type
    Yeast replicating
  • Selectable markers
    Lysine

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    lys5 flanked by loxp
  • Species
    S. cerevisiae (budding yeast)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSA40 was a gift from John McCusker (Addgene plasmid # 35114 ; http://n2t.net/addgene:35114 ; RRID:Addgene_35114)

  • For your References section:

    Positive and negative selection LYS5MX gene replacement cassettes for use in Saccharomyces cerevisiae. Ito-Harashima S, McCusker JH. Yeast. 2004 Jan 15;21(1):53-61. 10.1002/yea.1057 PubMed 14745782
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