ampkar
(Plasmid #35097)

• Purpose: A fluorescent reporter of AMPK activity and cellular energy stress
• Depositing Lab: Lewis Cantley
• Publication: Tsou et al Cell Metab. 2011 Apr 6;13(4):476-86.

Backbone

• Vector backbone: pcDNA3
• Backbone size w/o insert (bp): 5400
• Vector type: Bacterial Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: ampkar
• Species: artificial
• Insert Size (bp): 2300
• Mutation: na
• GenBank ID: na
• Promoter: CMV
• Tags / Fusion Proteins:
  • ECFP (N terminal on insert)
  • Venus (C terminal on insert)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: T7

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry