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Addgene

pcDNA Myc DBC1
(Plasmid #35096)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 35096 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA 3
  • Backbone manufacturer
    Invitrogen
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    deleted in breast cancer 1
  • Alt name
    DBC1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2769
  • Entrez Gene
    CCAR2 (a.k.a. DBC-1, DBC1, KIAA1967, NET35, p30 DBC, p30DBC)
  • Promoter CMV
  • Tag / Fusion Protein
    • Myc (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SP6
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA Myc DBC1 was a gift from Osamu Hiraike (Addgene plasmid # 35096 ; http://n2t.net/addgene:35096 ; RRID:Addgene_35096)

  • For your References section:

    Identification of DBC1 as a transcriptional repressor for BRCA1. Hiraike H, Wada-Hiraike O, Nakagawa S, Koyama S, Miyamoto Y, Sone K, Tanikawa M, Tsuruga T, Nagasaka K, Matsumoto Y, Oda K, Shoji K, Fukuhara H, Saji S, Nakagawa K, Kato S, Yano T, Taketani Y. Br J Cancer. 2010 Mar 16;102(6):1061-7. Epub 2010 Feb 16. 10.1038/sj.bjc.6605577 PubMed 20160719
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