pUC-mCA1-emptyMCS
(Plasmid
#35095)
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Purpose(Empty Backbone)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 35095 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUC
- Backbone size (bp) 2654
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Modifications to backbonemCA1 enhancer (243-2721) mCA1 colonic promoter (2967-13565) Multi cloning site (13566-13607) poly A signals (13608-14098)
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Vector typeMouse Targeting
- Promoter mouse carbonic anhydrase 1
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)XL10 Gold
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Copy numberUnknown
Resource Information
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Addgene Notes
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pUC-mCA1-emptyMCS was a gift from James Fleet (Addgene plasmid # 35095 ; http://n2t.net/addgene:35095 ; RRID:Addgene_35095) -
For your References section:
Generation of a transgenic mouse for colorectal cancer research with intestinal cre expression limited to the large intestine. Xue Y, Johnson R, Desmet M, Snyder PW, Fleet JC. Mol Cancer Res. 2010 Aug;8(8):1095-104. Epub 2010 Jul 27. 10.1158/1541-7786.MCR-10-0195 PubMed 20663863