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Addgene

BRCA1-(540-758)
(Plasmid #35071)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 35071 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET15b
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5700
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    BRCA1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    369
  • Mutation
    Contains aa 540-758
  • Entrez Gene
    BRCA1 (a.k.a. BRCAI, BRCC1, BROVCA1, FANCS, IRIS, PNCA4, PPP1R53, PSCP, RNF53)
  • Promoter T7
  • Tag / Fusion Protein
    • His (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7-term
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Protein expression in BL21DE3-Gold. Induce culture at OD600=0.8 with 0.5mM IPTG, 37oC for 3 hours.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    BRCA1-(540-758) was a gift from Cheryl Arrowsmith (Addgene plasmid # 35071 ; http://n2t.net/addgene:35071 ; RRID:Addgene_35071)

  • For your References section:

    Characterization of segments from the central region of BRCA1: an intrinsically disordered scaffold for multiple protein-protein and protein-DNA interactions?. Mark WY, Liao JC, Lu Y, Ayed A, Laister R, Szymczyna B, Chakrabartty A, Arrowsmith CH. J Mol Biol. 2005 Jan 14;345(2):275-87. 10.1016/j.jmb.2004.10.045 PubMed 15571721
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