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IFI16-(515-710)-HinB (pcDNA3-FLAG)
(Plasmid #35063)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 35063 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3-FLAG
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    IFI16-(515-710)-HinB
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    585
  • Mutation
    Contains HinB domain: aa 515-710
  • Entrez Gene
    IFI16 (a.k.a. IFNGIP1, PYHIN2)
  • Promoter CMV
  • Tag / Fusion Protein
    • FLAG (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer CMV-Fwd
  • 3′ sequencing primer BGH-Rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    IFI16-(515-710)-HinB (pcDNA3-FLAG) was a gift from Cheryl Arrowsmith (Addgene plasmid # 35063 ; http://n2t.net/addgene:35063 ; RRID:Addgene_35063)

  • For your References section:

    Interferon-inducible protein 16: insight into the interaction with tumor suppressor p53. Liao JC, Lam R, Brazda V, Duan S, Ravichandran M, Ma J, Xiao T, Tempel W, Zuo X, Wang YX, Chirgadze NY, Arrowsmith CH. Structure. 2011 Mar 9;19(3):418-29. 10.1016/j.str.2010.12.015 PubMed 21397192
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