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IFI16-(192-393)-HinA-Y218A-Y267A
(Plasmid #35059)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 35059 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET15b
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5700

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    induce culture at OD600=0.8 with 1mM IPTG, 15oC overnight
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    IFI16-(192-393)-HinA
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    603
  • Mutation
    Contains HinA domain: aa 192-393; Y218A and Y267A mutationson
  • Entrez Gene
    IFI16 (a.k.a. IFNGIP1, PYHIN2)
  • Promoter T7
  • Tags / Fusion Proteins
    • His (N terminal on backbone)
    • His (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7-term
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Growth conditions for protein production: BL21DE3-Codon-plus-RIL

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    IFI16-(192-393)-HinA-Y218A-Y267A was a gift from Cheryl Arrowsmith (Addgene plasmid # 35059 ; http://n2t.net/addgene:35059 ; RRID:Addgene_35059)

  • For your References section:

    Interferon-inducible protein 16: insight into the interaction with tumor suppressor p53. Liao JC, Lam R, Brazda V, Duan S, Ravichandran M, Ma J, Xiao T, Tempel W, Zuo X, Wang YX, Chirgadze NY, Arrowsmith CH. Structure. 2011 Mar 9;19(3):418-29. 10.1016/j.str.2010.12.015 PubMed 21397192
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