IFI16-(192-393)-HinA-E381A
(Plasmid
#35058)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 35058 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET15b
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Backbone manufacturerNovagen
- Backbone size w/o insert (bp) 5700
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsinduce culture at OD600=0.8 with 1mM IPTG, 15oC overnight
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameIFI16-(192-393)-HinA
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SpeciesH. sapiens (human)
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Insert Size (bp)603
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MutationContains HinA domain: aa 192-393; E381A mutation
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Entrez GeneIFI16 (a.k.a. IFNGIP1, PYHIN2)
- Promoter T7
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Tag
/ Fusion Protein
- His (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T7-term (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Growth conditions for protein production: BL21DE3-Codon-plus-RIL
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
IFI16-(192-393)-HinA-E381A was a gift from Cheryl Arrowsmith (Addgene plasmid # 35058 ; http://n2t.net/addgene:35058 ; RRID:Addgene_35058) -
For your References section:
Interferon-inducible protein 16: insight into the interaction with tumor suppressor p53. Liao JC, Lam R, Brazda V, Duan S, Ravichandran M, Ma J, Xiao T, Tempel W, Zuo X, Wang YX, Chirgadze NY, Arrowsmith CH. Structure. 2011 Mar 9;19(3):418-29. 10.1016/j.str.2010.12.015 PubMed 21397192