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Addgene

pMA3160
(Plasmid #35043)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 35043 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pMA2706
  • Backbone manufacturer
    Alexeyev's Lab
  • Backbone size w/o insert (bp) 6588
  • Vector type
    Mammalian Expression, Lentiviral, Luciferase
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    None
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Firefly luciferase
  • Alt name
    luc
  • Species
    Photinus pyralis
  • Insert Size (bp)
    1700
  • Promoter Tet

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer not available
  • 3′ sequencing primer not available
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    pGL3 Basic
  • Article Citing this Plasmid

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMA3160 was a gift from Mikhail Alexeyev (Addgene plasmid # 35043 ; http://n2t.net/addgene:35043 ; RRID:Addgene_35043)
  • For your References section:

    A retro-lentiviral system for doxycycline-inducible gene expression and gene knockdown in cells with limited proliferative capacity. Alexeyev MF, Fayzulin R, Shokolenko IN, Pastukh V. Mol Biol Rep. 2010 Apr . 37(4):1987-91. 10.1007/s11033-009-9647-7 PubMed 19655272