pNS207
(Plasmid
#35041)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 35041 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepUC 18
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namecytochrome b562
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Speciese. coli
- Promoter lac
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Sma 1 (not destroyed)
- 3′ cloning site Sal 1 (not destroyed)
- 5′ sequencing primer M13R
- 3′ sequencing primer M13 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pNS207 was a gift from Stephen Sligar (Addgene plasmid # 35041 ; http://n2t.net/addgene:35041 ; RRID:Addgene_35041) -
For your References section:
Cloning and expression of the gene encoding the soluble cytochrome b562 of Escherichia coli. Nikkila H, Gennis RB, Sligar SG. Eur J Biochem. 1991 Dec 5;202(2):309-13. 10.1111/j.1432-1033.1991.tb16377.x PubMed 1761034