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Addgene

pShuttle-FEN1deltaP
(Plasmid #35030)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 35030 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pShuttle
  • Backbone manufacturer
    Stratgene
  • Backbone size w/o insert (bp) 7500
  • Total vector size (bp) 9000
  • Vector type
    Mammalian Expression, Adenoviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL10 Gold
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FEN1DELTAP
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1500
  • Mutation
    8 AMINO ACID DELETION OF P BOX (amino acids 337–344)
  • Entrez Gene
    FEN1 (a.k.a. FEN-1, MF1, RAD2)
  • Promoter CMV
  • Tag / Fusion Protein
    • Flag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRV (destroyed during cloning)
  • 3′ cloning site EcoRV (destroyed during cloning)
  • 5′ sequencing primer GGTctatataagcagagctg
  • 3′ sequencing primer CTGatcataatcagccataccac
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pShuttle-FEN1deltaP was a gift from Sheila Stewart (Addgene plasmid # 35030 ; http://n2t.net/addgene:35030 ; RRID:Addgene_35030)

  • For your References section:

    FEN1 ensures telomere stability by facilitating replication fork re-initiation. Saharia A, Teasley DC, Duxin JP, Dao B, Chiappinelli KB, Stewart SA. J Biol Chem. 2010 Aug 27;285(35):27057-66. Epub 2010 Jun 15. 10.1074/jbc.M110.112276 PubMed 20551483
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