EGFP-Tpr
(Plasmid #35024)

• Depositing Lab: Larry Gerace
• Publication: Frosst et al J Cell Biol. 2002 Feb 18;156(4):617-30. Epub 2002 Feb 11.

Backbone

• Vector backbone: pEGFP-N1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): Stbl2
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Tpr
• Species: H. sapiens (human)
• Insert Size (bp): 7000
• Entrez Gene: TPR (a.k.a. MRT79)
• Promoter: CMV
• Tag / Fusion Protein:
  • EGFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (destroyed during cloning)
• 3′ cloning site: KpnI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: EGFP-N

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Tpr MUST be grown in STBL2 cells (Gibco/Invitrogen). Refer to manufacturer protocol that comes with the cells, grow everything at 30 degrees. Tpr seems to be recombinogenic, and STBL2 cells help with that.