pDS221
(Plasmid #34981)

• Purpose: Expresses a high-affinity ePDZ variant fused to mCherry in yeast. Illumination with 440 nm light induces binding to LOVpep.
• Depositing Lab: Michael Glotzer
• Publication: Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nmeth.1904.

Backbone

• Vector backbone: YIPlac211
• Modifications to backbone: PTEF–ePDZb1–mCherry–TCYC1 cassette inserted into YIPlac211
• Vector type: Yeast Expression
• Selectable markers: URA3

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ePDZb1–mCherry
• Species: S. cerevisiae (budding yeast)
• Promoter: TEF

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SacI (not destroyed)
• 3′ cloning site: KpnI (not destroyed)
• 5′ sequencing primer: M13 fwd
• 3′ sequencing primer: M13 rev

Resource Information

• Supplemental Documents:
  • Genbank sequence
• A portion of this plasmid was derived from a plasmid made by: Sequences from pGREG plasmids (EUROSCARF). ePDZ CDS from Shohei Koide, University of Chicago
• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry