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pDS190
(Plasmid #34977)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 34977 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGREG503
  • Backbone manufacturer
    EUROSCARF
  • Backbone size w/o insert (bp) 6624
  • Modifications to backbone
    PADH-ePDZ cassette inserted into pGREG503
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3 ; KanMX

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ePDZb
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    2128
  • Promoter ADH

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SacI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer M13 rev
  • 3′ sequencing primer M13 fwd
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Sequences from pGREG plasmids (EUROSCARF). ePDZ CDS from Shohei Koide, University of Chicago

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDS190 was a gift from Michael Glotzer (Addgene plasmid # 34977 ; http://n2t.net/addgene:34977 ; RRID:Addgene_34977)

  • For your References section:

    TULIPs: tunable, light-controlled interacting protein tags for cell biology. Strickland D, Lin Y, Wagner E, Hope CM, Zayner J, Antoniou C, Sosnick TR, Weiss EL, Glotzer M. Nat Methods. 2012 Mar 4. doi: 10.1038/nmeth.1904. 10.1038/nmeth.1904 PubMed 22388287
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