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Addgene

Ube1/PET21d
(Plasmid #34965)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 34965 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    PET21d
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL10 Gold
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Ube1
  • Alt name
    UBA1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3176
  • GenBank ID
    NM_153280
  • Entrez Gene
    UBA1 (a.k.a. A1S9, A1S9T, A1ST, AMCX1, CFAP124, GXP1, POC20, SMAX2, UBA1A, UBE1, UBE1X, VEXAS)
  • Promoter T7
  • Tag / Fusion Protein
    • His (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (not destroyed)
  • 3′ cloning site Not I (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7term
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Ube1/PET21d was a gift from Cynthia Wolberger (Addgene plasmid # 34965 ; http://n2t.net/addgene:34965 ; RRID:Addgene_34965)

  • For your References section:

    A spectrophotometric assay for conjugation of ubiquitin and ubiquitin-like proteins. Berndsen CE, Wolberger C. Anal Biochem. 2011 Nov 1;418(1):102-10. Epub 2011 Jul 2. 10.1016/j.ab.2011.06.034 PubMed 21771579
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