pPSmOrange2-Tubulin
(Plasmid #34963)

• Depositing Lab: Vladislav Verkhusha
• Publication: Subach et al J Am Chem Soc. 2012 Aug 17.

Backbone

• Vector backbone: pC1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 5328
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PSmOrange2
• Mutation: R17H, R36H, F64I, Q194L, A224S, G226A (mutations are relative to PSmOrange but numbering is relative to EGFP)
• GenBank ID: JQ392581 NM_001018942
• Tag / Fusion Protein:
  • Tubulin (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: BglII (not destroyed)
• 5′ sequencing primer: 5'-cggtaggcgtgtacggtgggag
• 3′ sequencing primer: 5'-gttcagggggaggtgtgggagg

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

In Addgene's CMV-F sequencing result, there is a 6 nucleotide deletion corresponding to position 595 of the full plasmid sequence. It is before the ORF and does not appear alter any restriction sites or the function of the plasmid.
https://www.ncbi.nlm.nih.gov/nuccore/JQ392581