pKeratin-PSmOrange2
(Plasmid #34962)

• Depositing Lab: Vladislav Verkhusha
• Publication: Subach et al J Am Chem Soc. 2012 Aug 17.

Backbone

• Vector backbone: pN1
• Backbone size w/o insert (bp): 5275
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PSmOrange2
• Insert Size (bp): 711
• Mutation: R17H, R36H, F64I, Q194L, A224S, G226A (mutations are relative to PSmOrange but numbering is relative to EGFP)
• GenBank ID: JQ392581 NM_001018942
• Tag / Fusion Protein:
  • Keratin (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: 5'-cggtaggcgtgtacggtgggag
• 3′ sequencing primer: 5'-gttcagggggaggtgtgggagg

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

https://www.ncbi.nlm.nih.gov/nuccore/JQ392581

In Addgene's SV40pA-R sequencing result, there is one mismatch and a two nucleotide deletion corresponding to position 2652 of the full plasmid sequence. This results in a loss of the NotI site but does not alter the function of the plasmid.