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pCS2+8NmCherry-ABCB4a
(Plasmid #34941)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 34941 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCS2+8NmCherry
  • Backbone manufacturer
    Gokirmak et al., 2012
  • Backbone size w/o insert (bp) 4851
  • Vector type
    Mammalian Expression ; Sea urchin, zebrafish, Xenopus

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH10B
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ABCB4a
  • Alt name
    MDR3
  • Species
    Strongylocentrotus purpuratus
  • Insert Size (bp)
    3897
  • Promoter SP6
  • Tag / Fusion Protein
    • mCherry (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site FseI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CTTGAAGCTGTCCTTCCCCGA
  • 3′ sequencing primer TGTTGTTAACTTGTTTATTGCAGCT
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCS2+8NmCherry-ABCB4a was a gift from Amro Hamdoun (Addgene plasmid # 34941 ; http://n2t.net/addgene:34941 ; RRID:Addgene_34941)

  • For your References section:

    Localization and Substrate Selectivity of Sea Urchin Multidrug (MDR) Efflux Transporters. Gokirmak T, Campanale JP, Shipp LE, Moy GW, Tao H, Hamdoun A. J Biol Chem. 2012 Nov 2. 10.1074/jbc.M112.424879 PubMed 23124201
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