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pT5T_hp53_ST_CR2
(Plasmid #34923)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 34923 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pT5T
  • Backbone size w/o insert (bp) 5194
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    p53 DNA-binding (Res 94-358, deletion 293-321)
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    713
  • Mutation
    C135V, C141V, W146Y, C182S, V203A, R209P, C229Y, H233Y, Y234F, N235K, Y236F, T253V, N268D, P322T, L323M, M340Q, L344R, G356T, and K357E
  • Entrez Gene
    TP53 (a.k.a. BCC7, BMFS5, LFS1, P53, TRP53)
  • Promoter T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (unknown if destroyed)
  • 3′ cloning site HindIII (unknown if destroyed)
  • 5′ sequencing primer GGATCC
  • 3′ sequencing primer AAGCTT
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pT5T_hp53_ST_CR2 was a gift from Thanos Halazonetis (Addgene plasmid # 34923 ; http://n2t.net/addgene:34923 ; RRID:Addgene_34923)

  • For your References section:

    An induced fit mechanism regulates p53 DNA binding kinetics to confer sequence specificity. Petty TJ, Emamzadah S, Costantino L, Petkova I, Stavridi ES, Saven JG, Vauthey E, Halazonetis TD. EMBO J. 2011 Jun 1;30(11):2167-76. Epub 2011 Apr 26. 10.1038/emboj.2011.127 PubMed 21522129
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