pBacPuroR-NeoR
(Plasmid #34921)

• Depositing Lab: Ben Lehner
• Publication: Semple et al Nat Methods. 2012 Jan 30;9(2):118-9. doi: 10.1038/nmeth.1864.

Backbone

• Vector backbone: pPD49.78
• Backbone size w/o insert (bp): 3300
• Modifications to backbone: PuroR and NeoR under the control of 5' and 3' bacterial elements taken from up and downstream of the Ampicillin resistance gene.
• Vector type: Bacterial Expression
• Selectable markers: Neomycin (select with G418), Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: Bacterial promoter::PuroR::bacterial 3p flank
• Alt name: Puromycin N-acetyl-tranferase (PAC)
• Species: Streptomyces
• Insert Size (bp): 979

Gene/Insert 2

• Gene/Insert name: Bacterial promoter::NeoR::bacterial 3p flank
• Alt name: neo
• Species: Tn5 transposon
• Insert Size (bp): 1170

Resource Information

• Supplemental Documents:
  • GenBank file
• A portion of this plasmid was derived from a plasmid made by: Based on pPD49.78 vector (Addgene). Puromycin resistance gene from pBabePuro (Addgene). 5' and 3' sequences flanking the AmpR gene were placed upstream and downstream of PuroR and NeoR to confer bacterial expression
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Transform this plasmid into E. coli and use as a food source for worms grown on puromycin & G418 selective plates.