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paavCAG-pre-mGRASP-mCerulean-2A-nls-mCherry
(Plasmid #34911)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 34911 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    paavCAG
  • Backbone size w/o insert (bp) 5000
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    presynaptic mGRASP
  • Alt name
    pre-mGRASP
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1722
  • GenBank ID
    JN898961
  • Promoter CAG

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer gcggctctagagcctctgcta
  • 3′ sequencing primer ttaaagcagcgtatccacat
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    paavCAG-pre-mGRASP-mCerulean-2A-nls-mCherry was a gift from Jinhyun Kim (Addgene plasmid # 34911 ; http://n2t.net/addgene:34911 ; RRID:Addgene_34911)

  • For your References section:

    mGRASP enables mapping mammalian synaptic connectivity with light microscopy. Kim J, Zhao T, Petralia RS, Yu Y, Peng H, Myers E, Magee JC. Nat Methods. 2011 Dec 4;9(1):96-102. doi: 10.1038/nmeth.1784. 10.1038/nmeth.1784 PubMed 22138823
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