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Ai38
(Plasmid #34883)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 34883 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Rosa26 Targeting Vector
  • Backbone size w/o insert (bp) 13978
  • Vector type
    Mouse Targeting
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    Stbl2
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CAG-Floxed GCaMP3
  • Insert Size (bp)
    1353
  • Promoter CAG

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site FseI (not destroyed)
  • 3′ cloning site FseI (not destroyed)
  • 5′ sequencing primer pCAG-F, Xpress_fwd
  • 3′ sequencing primer MSCV-rev
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Backbone - Liqun Luo; GCaMP3 – L. Tian and L.L. Looger
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Ai38 was a gift from Hongkui Zeng (Addgene plasmid # 34883 ; http://n2t.net/addgene:34883 ; RRID:Addgene_34883)

  • For your References section:

    A Cre-dependent GCaMP3 reporter mouse for neuronal imaging in vivo. Zariwala HA, Borghuis BG, Hoogland TM, Madisen L, Tian L, De Zeeuw CI, Zeng H, Looger LL, Svoboda K, Chen TW. J Neurosci. 2012 Feb 29;32(9):3131-41. doi: 10.1523/JNEUROSCI.4469-11.2012. 10.1523/JNEUROSCI.4469-11.2012 PubMed 22378886
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