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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 34881 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneRosa26 Targeting Vector
- Backbone size w/o insert (bp) 13898
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Vector typeMouse Targeting
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)Stbl2
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCAG-Floxed Synaptophysin-tdTomato
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Insert Size (bp)2361
- Promoter CAG
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Tag
/ Fusion Protein
- tdTomato (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site MluI (not destroyed)
- 3′ cloning site MluI (not destroyed)
- 5′ sequencing primer pCAG-F, dsRed1-C
- 3′ sequencing primer MSCV-rev (Common Sequencing Primers)
Resource Information
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Addgene Notes
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A portion of this plasmid was derived from a plasmid made byBackbone - Liqun Luo
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Ai34 was a gift from Hongkui Zeng (Addgene plasmid # 34881 ; http://n2t.net/addgene:34881 ; RRID:Addgene_34881) -
For your References section:
A toolbox of Cre-dependent optogenetic transgenic mice for light-induced activation and silencing. Madisen L, Mao T, Koch H, Zhuo JM, Berenyi A, Fujisawa S, Hsu YW, Garcia AJ 3rd, Gu X, Zanella S, Kidney J, Gu H, Mao Y, Hooks BM, Boyden ES, Buzsaki G, Ramirez JM, Jones AR, Svoboda K, Han X, Turner EE, Zeng H. Nat Neurosci. 2012 Mar 25;15(5):793-802. doi: 10.1038/nn.3078. 10.1038/nn.3078 PubMed 22446880