Ai34
(Plasmid #34881)

• Depositing Lab: Hongkui Zeng
• Publication: Madisen et al Nat Neurosci. 2012 Mar 25;15(5):793-802. doi: 10.1038/nn.3078.

Backbone

• Vector backbone: Rosa26 Targeting Vector
• Backbone size w/o insert (bp): 13898
• Vector type: Mouse Targeting
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): Stbl2
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CAG-Floxed Synaptophysin-tdTomato
• Insert Size (bp): 2361
• Promoter: CAG
• Tag / Fusion Protein:
  • tdTomato (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: MluI (not destroyed)
• 3′ cloning site: MluI (not destroyed)
• 5′ sequencing primer: pCAG-F, dsRed1-C
• 3′ sequencing primer: MSCV-rev

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Digest
• A portion of this plasmid was derived from a plasmid made by: Backbone - Liqun Luo
• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry