Ai32
(Plasmid #34880)

• Purpose: Cre-dependent expression of ChR2(H134R)-EYFP, for optogenetic excitation
• Depositing Lab: Hongkui Zeng
• Publication: Madisen et al Nat Neurosci. 2012 Mar 25;15(5):793-802. doi: 10.1038/nn.3078.

Backbone

• Vector backbone: Rosa26 Targeting Vector
• Backbone size w/o insert (bp): 15567
• Vector type: Mouse Targeting
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): Stbl2
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Floxed ChR2(H134R)-EYFP
• Species: Synthetic
• Insert Size (bp): 1671
• Mutation: H134R in ChR2
• Promoter: CAG
• Tag / Fusion Protein:
  • EYFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: MluI (destroyed during cloning)
• 3′ cloning site: MluI (destroyed during cloning)
• 5′ sequencing primer: pCAG-F, EGFP_C
• 3′ sequencing primer: MSCV_rev

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Backbone - Liqun Luo; ChR2(H134R)-G. Nagel and A. Gottschalk
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry