pCJF604 - pDESTR4-R3(ttTi4391, I)
(Plasmid
#34861)
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 34861 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepDESTR4-R3
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Vector typeWorm targeting
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Growth instructionsIt is important to grow the bacteria under BOTH Amp and Cm selection.
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namettTi4391 targeting
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SpeciesC. elegans (nematode)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer M13F
- 3′ sequencing primer M13R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
There is a 461 bp deletion in the middle of the left recombination region. This deletion does not appear to adversely affect the ability to insert transgenes into the ttTi4391 site.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCJF604 - pDESTR4-R3(ttTi4391, I) was a gift from Erik Jorgensen (Addgene plasmid # 34861 ; http://n2t.net/addgene:34861 ; RRID:Addgene_34861) -
For your References section:
Improved Mos1-mediated transgenesis in C. elegans. Frokjaer-Jensen C, Davis MW, Ailion M, Jorgensen EM. Nat Methods. 2012 Jan 30;9(2):117-8. doi: 10.1038/nmeth.1865. 10.1038/nmeth.1865 PubMed 22290181
Map uploaded by the depositor.
