pTY1048-S65T-LacI
(Plasmid
#34853)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 34853 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepTY1048
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Vector typeWorm Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameGFP-LacI
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MutationGFP is mutated: S65T and contains introns, in order to increase expression of the fusion protein
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site None (unknown if destroyed)
- 3′ cloning site None (unknown if destroyed)
- 5′ sequencing primer n/a
- 3′ sequencing primer lacI-R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTY1048-S65T-LacI was a gift from Paul Sternberg (Addgene plasmid # 34853 ; http://n2t.net/addgene:34853 ; RRID:Addgene_34853) -
For your References section:
Visualization of C. elegans transgenic arrays by GFP. Gonzalez-Serricchio AS, Sternberg PW. BMC Genet. 2006 Jun 7;7:36. 10.1186/1471-2156-7-36 PubMed 16759392